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Grow P.ultimum on Malt Agar
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Period: to
Preparations
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Grow P. ultimum on Sorghum
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Grow biocontrol bacteria on agar plates
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Pregermination of cucumber seedlings: surface sterilization of seeds; incubate seeds on water agar in the dark
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Grow biocontrol bacteria in liquid culture
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Set up experiment: add pathogen to the soil, plant 3 pregerminated cucumber seedlings per pot and water pots with bacteria or control as well as with water
add pathogen to the soil, plant 3 pregerminated cucumber seedlings per pot and water pots with bacteria or control as well as with water -
Period: to
Running of experiment
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water plants
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water plants
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water plants
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water plants
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Harvest of experiment
assessing seedlings for survival/ scoring/ fresh weight
