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He used uncovered and covered jars with flesh to show that worms only appeared in uncovered jars where flies could lay eggs.
Contribution: Questioned the idea of spontaneous generation of larger organisms. -
He boiled a jar with bacteria to sterilize and kill them and observe the spontaneous creation of new microorganisms
Conclusion: supported the spontaneous generation of microorganisms. -
The nutrient broth was cooked in closed and open flasks, and it was observed that no microorganisms appeared in the closed flasks.
Contribution: Provides evidence against the spontaneous generation of microorganisms. -
He designed the swan-neck flask with S-curved necks, where he cooked the broth, curved-necked flasks to prevent the entry of microorganisms. He don't observe life for a year, since he expose the flask to the air, when the forms start appearing
Conclusion: Spontaneous generation successfully refuted.