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Seed sterilization
Seeds of Arabidopsis thaliana need to be sterilized with sodium hypochlorid and subsequently stored at 4 °C to synchronyze germination -
Seeds in growth chamber
Seeds are set in pots filled with soil and put into a tightly controlled growth chamber in order to initiate germination -
Transplanting the seedlings
Single Arabidopsis thaliana seedlings are transplanted into pots with exactly 60g sieved soil. Pots are gonna be watered for 25 minutes, covered and put back under controlled conditions in the green house -
Replace seedlings
A weeks after the cover will be removed. Young plants which suffered during the transplanting process will be replaced. Normally those are just few plants -
Watering
Plants were not watered since the trtansfer in single pots. Pots contain approximately 50g soil at this stage. They are gonna be watered for 5 minutes -
T0 sample
This will be the first time point at which plant material is harvested and weighted. The pots still contain 30g soil.At each time point the relative water content was calculated (8 replicates per genotype) -
T5 sample
This is the second selection point. Again plant material is harvested, weighted and relative water content will be determined -
Period: to
T7-11 samples
Further time point at which plant material is harvested, weighted and the relative water content is determined -
Rewatering
Plants are gonna be rewatered. After rewatering the survival rate was calculated as well -
Rewatering
Plants are gonna be rewatered. After rewatering the survival rate was calculated as well
